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Christensen Citrate Sulfide Medium

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

NaCl....................................................................5.0g

Sodium citrate·2H2O...........................................3.0g

KH2HPO4............................................................1.0g

Yeast extract........................................................0.5g

Ferric citrate........................................................0.2g

Ammonium citrate ..............................................0.2g

Glucose ...............................................................0.2g

L-Cysteine·HCl·H2O ...........................................0.1g

Na2S2O3·5H2O ..................................................0.08g

Phenol Red......................................................0.012g

pH 6.7± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Dispense

into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave

in tubes. Allow tubes to cool in a slanted position.

Use: For the differentiation of enteric pathogens, es

pecially members of the Enterobacteriaceae, and

coliforms based on their ability to utilize citrate as a

carbon source and production of H2S. Bacteria that can

utilize citrate turn the medium pink-red. H2S produc

tion appears as a blackening of the butt of the tube.