培养基配方

您现在的位置：网站首页 >> 培养基配方

Christensen’s Urea Agar with NaCl (BAM M40)

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Christensen’s Urea Agar with NaCl

(BAM M40)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Agar ..................................................................15.0g

KH2PO4...............................................................2.0g

Peptone ...............................................................1.0g

Glucose ...............................................................1.0g

Phenol Red......................................................0.012g

Urea solution............................................... 100.0mL

pH 6.8 ± 0.1 at 25°C

Urea Solution:

Composition per 100.0mL:

Urea...................................................................20.0g

Preparation of Urea Solution: Add urea to

100.0mL of distilled/deionized water. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, ex

cept urea solution, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50–55°C. Aseptically

add 100.0mL of sterile urea solution. Mix thorough

ly. Pur into Petri dishes or distribute into sterile tubes.

Allow tubes to solidify in a slanted position.

Use: For the differentiation of halophilic Vibrio spp.

on the basis of urease production.