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Clostridium acidurici Medium

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Uric acid..............................................................2.0g

Yeast extract........................................................1.0g

K2HPO4.............................................................0.91g

KOH..................................................................0.67g

Sodium thioglycolate..........................................0.5g

MgSO4·7H2O....................................................0.25g

CaCl2·2H2O ....................................................0.015g

FeSO4·7H2O.....................................................6.0mg

NaHCO3 solution .......................................... 25.0mL

Sodium thioglycolate solution ...................... 25.0mL

pH 7.0–7.5 at 25°C

NaHCO3 Solution:

Composition per 25.0mL:

NaHCO3..............................................................5.0g

Preparation of NaHCO3 Solution: Add

NaHCO3 to distilled/deionized water and bring vol

ume to 25.0mL. Mix thoroughly. Filter sterilize.

Sodium Thioglycolate Solution:

Composition per 25.0mL:

Sodium thioglycolate..........................................0.5g

Preparation of Sodium Thioglycolate Solu

tion: Add sodium thioglycolate to distilled/deion

ized water and bring volume to 25.0mL. Mix

thoroughly. Autoclave solution separately for 15 min

at 15 psi pressure–121°C.

Preparation of Medium: Add K2HPO4 and KOH

to distilled/deionized water and bring volume to 1.0L.

Add uric acid. Gently heat until boiling. Add the re

maining components, except NaHCO3 and sodium

thioglycolate. Mix thoroughly. Adjust pH to 7.0–7.5.

Distribute into tubes or flasks. Autoclave for 15 min at

15 psi pressure–121°C. Add 0.25mL of sterile

NaHCO3 solution and 0.25mL of sterile sodium

thioglycolate solution for each 10.0mL of sterile basal

medium.

Use: For the cultivation and maintenance of

Clostridium acidurici, Clostridium purinolyticum,

and other bacteria that can utilize uric acid as a car

bon source.