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Coliform Agar ES, Chromocult (Chromocult Coliform Agar ES) (Chromocult Enhanced Selectivity Agar)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Coliform Agar ES, Chromocult

(Chromocult Coliform Agar ES)

(Chromocult Enhanced Selectivity

Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

MOPS ...............................................................10.0g

KCl......................................................................7.5g

Peptone ..............................................................5.0g

Bile salts............................................................1.15g

Na-propionate .....................................................0.5g

6-Chloro-3-indoxyl-β-D-galactopyranoside ....0.15g

5-Bromo-4-chloro-3-indoxyl-

β-D-glucuronic acid .....................................0.1g

Isopropyl-β-D-thiogalactopyranoside ................0.1g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly and heat with frequent agitation until

components are completely dissolved (approximate

ly 45 min). Do not autoclave. Cool to 45°–50°C.

Pour into sterile Petri dishes. The plates should be

clear and colorless.

Use: For the detection of E. coli and total coliforms.

The combination of suitable peptones and the buffer

ing using MOPS allow rapid growth of coliforms and

an optimal transformation of the chromogenic sub

strates. The amount of bile salts and propionate large

ly inhibit growth of Gram-positive and Gram-nega

tive accompanying flora. The simultaneous detection

of total coliforms and E. coli is achieved using the

combination of two chromogrenic substrates. The

substrate Salmon™-β-D-GAL is split by β-D-galac

tosidase, characteristic for coliforms, resulting in a

salmon to red coloration of coliform colonies. The

detection of the β-D-glucuronidase, characteristic for

E. coli, is cleaved via the substrate X-β-D-glucu

ronide, causing a blue coloration of positive colonies.

As E. coli splits Salmon-β-D-GAL as well as X-β-D

glucuronide, the colonies turn to a dark violet color

and can be easily differentiated from the other

coliforms being salmon-red.

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Coliform Agar ES, Chromocult (Chromocult Coliform Agar ES) (Chromocult Enhanced Selectivity Agar)