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Cooked Meat Medium (BAM M42)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Cooked Meat Medium

(BAM M42)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart........................................................454.0g

Proteose peptone...............................................20.0g

NaCl....................................................................5.0g

Glucose ...............................................................2.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Finely chop beef heart.

Add approximately 1.5g of heart particles to test tubes.

Add remaining components to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Distrib

ute into tubes in 10.0mL volumes. Autoclave for 15

min at 15 psi pressure–121°C. Slowly cool tubes to

prevent expulsion of meat particles. Steam the steril

ized medium and cool, without agitation, just before

use.

Use: For the cultivation and maintenance of anaero

bic microorganisms.