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Cornmeal Agar (CMA)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Cornmeal Agar

(CMA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Cornmeal polenta..............................................15.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add Cornmeal polenta

to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat and bring to boil

ing. Continue boiling for 30 min. Filter through

Whatman #1 filter paper. Add agar to filtrate. Gently

heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 10 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of many

filamentous fungi.