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CreDm1 Medium

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

CreDm1 Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

Solution A................................................... 980.0mL

Solution D..................................................... 10.0mL

Solution E ..................................................... 10.0mL

Solution B ....................................................... 1.0mL

Solution C ....................................................... 1.0mL

pH 6.7–6.9 at 25°C

Solution A:

Composition per 980.0mL:

KH2PO4...............................................................1.4g

NH4Cl .................................................................0.5g

MgCl2·6H2O .......................................................0.2g

CaCl2·2H2O ......................................................0.15g

Yeast extract...................................................50.0mg

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

980.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Solution B (Trace Elements Solution SL-10):

Composition per liter:

FeCl2·4H2O.........................................................1.5g

CoCl2·6H2O .................................................190.0mg

MnCl2·4H2O ................................................100.0mg

ZnCl2..............................................................70.0mg

Na2MoO4·2H2O .............................................36.0mg

NiCl2·6H2O....................................................24.0mg

H3BO3 ..............................................................6.0mg

CuCl2·2H2O .....................................................2.0mg

HCl (25% solution)....................................... 10.0mL

Preparation of Solution B (Trace Elements

Solution SL-10): Add FeCl2·4H2O to 10.0mL of

HCl solution. Mix thoroughly. Add distilled/deion

ized water and bring volume to 1.0L. Add remaining

components. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C.

Solution C (Selenite-Tungstate Solution):

Composition per liter:

NaOH..................................................................0.5g

Na2WO4·2H2O.................................................4.0mg

Na2SeO3·5H2O ................................................3.0mg

Preparation of Solution C (Selenite-Tung

state Solution): Add components to distilled/

deionized water and bring volume to 1.0L. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Solution D (Vitamin Solution):

Composition per liter:

Pyridoxine·HCl ..............................................10.0mg

Calcium DL-pantothenate.................................5.0mg

Lipoic acid .......................................................5.0mg

Nicotinic acid...................................................5.0mg

p-Aminobenzoic acid.......................................5.0mg

Riboflavin ........................................................5.0mg

Thiamine·HCl ..................................................5.0mg

Biotin ...............................................................2.0mg

Folic acid..........................................................2.0mg

Vitamin B12 ......................................................0.1mg

Preparation of Solution D (Vitamin Solution):

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Filter steril

ize.

Solution E:

Composition per 10.0mL:

Disodium-DL-malate ...........................................1.6g

Preparation of Solution E: Add disodium-DL

malate to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Preparation of Medium: Aseptically combine

980.0mL of sterile solution A with 1.0mL of sterile

solution B, 1.0mL of sterile solution C, 10.0mL of

sterile solution D, and 10.0mL of sterile solution E,

in that order. Mix thoroughly. Adjust pH to 6.7–6.9.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Campylobacter species.

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CreDm1 Medium