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Deoxycholate Citrate Agar

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium citrate...................................................50.0g

Agar ..................................................................15.0g

Lactose..............................................................10.0g

Beef extract.........................................................5.0g

Peptone ...............................................................5.0g

Na2S2O3·5H2O ....................................................5.0g

Sodium deoxycholate..........................................2.5g

Ferric citrate........................................................1.0g

Neutral Red.....................................................0.025g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed pow

der from Oxoid.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Do not au

toclave. Cool to 45°–50°C. Pour into sterile Petri dishes.

Dry the agar surface before use.

Use: For the selective isolation and cultivation of en

teric pathogens, especially Salmonella and Shigella

species.