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Deoxycholate Lactose Agar

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue .............................5.0g

Sodium citrate.....................................................2.0g

Sodium deoxycholate..........................................0.5g

Neutral Red.....................................................0.033g

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed pow

der from BD Diagnostic Systems.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Do not au

toclave. Cool to 45°–50°C. Pour into sterile Petri dishes.

Dry the agar surface before use.

Use: For the selective isolation, cultivation, and differ

entiation of enteric pathogens, especially Salmonella

and Shigella species. Lactose-fermenting bacteria ap

pear as pink colonies that may or may not be surrounded

by a zone of precipitated deoxycholate. Nonlactose-fer

menting bacteria appear as colorless colonies that are

surrounded by a clear orange-yellow zone. Used for the

enumeration of coliform bacteria from milk and dairy

products.