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Eagle’s Minimal Essential Medium with Earle’s Salts and Nonessential Amino Acids (MEM with Earle’s Salts and Nonessential Amino Acids) (BAM M46)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Eagle’s Minimal Essential Medium

with Earle’s Salts

and Nonessential Amino Acids

(MEM with Earle’s Salts

and Nonessential Amino Acids)

(BAM M46)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl....................................................................6.8g

NaHCO3..............................................................2.2g

Glucose ...............................................................1.0g

KCl......................................................................0.4g

CaCl2·2H2O ....................................................0.265g

MgSO4·7H2O......................................................0.2g

L-Arginine·H2O.................................................0.15g

NaH2PO4·H2O...................................................0.14g

L-Arginine·HCl................................................0.126g

L-Lysine·HCl ..................................................72.5mg

L-Tyrosine, disodium salt...............................52.1mg

L-Leucine .......................................................52.0mg

L-Threonine....................................................48.0mg

L-Valine ..........................................................46.0mg

L-Histidine·HCl·H2O......................................42.0mg

D-Phenylalanine .............................................32.0mg

L-Cysteine·2HCl...........................................31.29mg

L-Methionine..................................................15.0mg

L-Glutamic acid..............................................14.7mg

L-Aspartic acid ...............................................13.3mg

L-Proline.........................................................11.5mg

L-Serine ..........................................................10.5mg

L-Tryptophan..................................................10.0mg

L-Alanine..........................................................8.9mg

Phenol Red.....................................................10.0mg

L-Glycine..........................................................7.5mg

i-Inositol...........................................................2.0mg

D-Calcium pantothenate ...................................1.0mg

Choline chloride...............................................1.0mg

Folic acid .........................................................1.0mg

Nicotinamide....................................................1.0mg

Pyridoxal·HCl ..................................................1.0mg

Thiamine·HCl ..................................................1.0mg

Riboflavin ........................................................0.1mg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

1.0L of distilled/deionized water. Mix thoroughly.

Filter sterilize.

Use: For the cultivation of animal cells in tissue cul

ture, for example cells for viral detection and identi

fication by characteristic cytopathic effects.

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Eagle’s Minimal Essential Medium with Earle’s Salts and Nonessential Amino Acids (MEM with Earle’s Salts and Nonessential Amino Acids) (BAM M46)