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EC Broth (Escherichia coli Broth) (EC Medium) (BAM M49)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

EC Broth

(Escherichia coli Broth)

(EC Medium)

(BAM M49)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Lactose................................................................5.0g

NaCl....................................................................5.0g

K2HPO4...............................................................4.0g

KH2PO4...............................................................1.5g

Bile salts No.3...................................................1.12g

Novobiocin solution...................................... 10.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Novobiocin Solution:

Composition per 50.0mL:

Novobiocin .........................................................0.1g

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

50°C. Aseptically add 10.0mL novobiocin solution.

Mix thoroughly. Aseptically distribute to sterile

tubes or flasks.

Use: A selective enrichment broth for the growth of

E. coli O157, from food and environmental samples.