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EC Medium, Modified with Novobiocin

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone............................................................20.0g

NaCl....................................................................5.0g

Lactose................................................................5.0g

K2HPO4...............................................................4.0g

KH2PO4...............................................................1.5g

Bile salts............................................................1.12g

Novobiocin supplement ................................ 10.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder and supplement from BD Diagnostic Sys

tems.

Novobiocin Supplement:

Composition per 10.0mL:

Sodium novobiocin........................................20.0mg

Preparation of Novobiocin Supplement: Add

Sodium novobiocin to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept novobiocin supplement, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Aseptically add 10.0mL of sterile novobiocin supple

ment. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks.

Use: For the cultivation of Escherichia coli O157:H7.