培养基配方

您现在的位置：网站首页 >> 培养基配方

EE Broth, Mossel (Enterobacteriaceae Enrichment Broth, Mossel)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

EE Broth, Mossel

(Enterobacteriaceae

Enrichment Broth, Mossel)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Enzymatic hydrolysate of protein.....................10.0g

Na2HPO4.............................................................8.0g

Glucose ...............................................................5.0g

KH2PO4...............................................................2.0g

Oxgall .................................................................0.1g

Brilliant Green ..............................................0.0135g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into flasks in 120.0mL

volumes. Gently heat at 100°C for 30 min. Do not au

toclave. Cool rapidly to 25°C.

Use: For the cultivation and enrichment of members

of the Enterobateriaceae in the examination of foods

and animal feed. Used in conjunction with tryptone

soy broth. Bacteria belonging to the Enterobacteri

aceae turn this medium turbid and yellow-green.