培养基配方

您现在的位置：网站首页 >> 培养基配方

EMB Agar (Eosin Methylene Blue Agar)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

EMB Agar

(Eosin Methylene Blue Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.5g

Pancreatic digest of casein................................10.0g

Lactose................................................................5.0g

Sucrose................................................................5.0g

K2HPO4...............................................................2.0g

Eosin Y ...............................................................0.4g

Methylene Blue...............................................0.065g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es.

Use: For the isolation, cultivation, and differentia

tion of Gram-negative enteric bacteria based on lac

tose fermentation. Bacteria that ferment lactose,

especially the coliform bacterium Escherichia coli,

appear as colonies with a green metallic sheen or

blue-black to brown color. Bacteria that do not fer

ment lactose appear as colorless or transparent, light

purple colonies.