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MacConkey Agar No. 2 (MacConkey II Agar)

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Agar No. 2

(MacConkey II Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................20.0g

Agar ..................................................................15.0g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Bile salts No. 2....................................................1.5g

Neutral Red.......................................................0.05g

Crystal Violet ...................................................1.0mg

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring until boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the selective isolation, cultivation, and dif

ferentiation of enteric pathogens, especially entero

cocci, in clinical specimens and in materials of sani

tary importance.