培养基配方

您现在的位置：网站首页 >> 培养基配方

MacConkey Agar without Crystal Violet

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Lactose..............................................................10.0g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue ...........................10.0g

Bile salts..............................................................5.0g

NaCl....................................................................5.0g

Neutral Red.......................................................0.05g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring until boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the detection of members of the Enterobac

teriaceae and enterococci as well as some staphylo

cocci. For the isolation and detection of coliforms

and enteric pathogens from water and wastewater.