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MacConkey Broth

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................20.0g

Lactose..............................................................10.0g

Bile salts..............................................................5.0g

NaCl....................................................................5.0g

Neutral Red.....................................................0.075g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

If testing 10.0mL samples, prepare medium double

strength. Mix thoroughly. Gently heat while stirring

until boiling. Distribute into test tubes containing in

verted Durham tubes. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the selective isolation and cultivation of

coliforms in milk and water.