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Malt Extract Agar

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

Malt Extract Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Malt extract.......................................................30.0g

Agar ..................................................................15.0g

Mycological peptone...........................................5.0g

pH 5.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring until boil

ing. Distribute into tubes or flasks. Autoclave for 10

min at 15 psi pressure–115°C. Do not overheat or

agar will not harden. If a lower pH (3.5) is desired,

cool medium to 55°C and aseptically add 100.0mL of

sterile lactic acid. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the detection, isolation, and enumeration of

yeasts and molds. The addition of lactic acid sup

presses bacterial growth.