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Mannitol Egg Yolk Polymyxin Agar (MYP Agar)

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Egg Yolk

Polymyxin Agar

(MYP Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

D-Mannitol ........................................................10.0g

Peptone .............................................................10.0g

NaCl..................................................................10.0g

Beef extract.........................................................1.0g

Phenol Red......................................................0.025g

Egg yolk emulsion, 50%............................... 50.0mL

Polymyxin B ................................................. 10.0mL

pH 7.2 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks.........................................variable

NaCl (0.85% solution) .................................. 40.0mL

Preparation of Egg Yolk Emulsion: Wash fresh

eggs wtih stiff brush and drain. Soak eggs in 70%

ethanol for 1 hr. Crack eggs aseptically and separate

yolks from whites. Remove egg yolks with a sterile

syringe or a wide-mouth pipet. Place 50.0mL of egg

yolks into a sterile container. Add 50.0mL sterile

0.85% saline.

Polymyxin B Solution:

Composition per 50.0mL:

Polymyxin B ..............................................500,000U

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept egg yolk emulsion, 50%, and polymyxin B solu

tion—to distilled/deionized water and bring volume

to 940.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 49°C. Aseptically add 50.0mL of

sterile egg yolk emulsion, 50%, and 10.0mL of sterile

polymyxin B solution. Mix thoroughly. Pour into

sterile Petri dishes.

Use: For the cultivation and enumeration of Bacillus

cereus from foods.

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Mannitol Egg Yolk Polymyxin Agar (MYP Agar)