培养基配方

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McBride Agar, Modified

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glycine anhydride.............................................10.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Phenylethanol......................................................2.5g

LiCl .....................................................................0.5g

Cycloheximide solution ................................ 10.0mL

pH 7.3 ± 0.2 at 25°C

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.2g

Preparation of Cycloheximide Solution: Add

cycloheximide to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept cycloheximide solution, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile cycloheximide solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation of Listeria monocytogenes

from dairy products.