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McClung-Toabe Agar

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

McClung-Toabe Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone...............................................40.0g

Agar ..................................................................25.0g

Na2HPO4.............................................................5.0g

Glucose ...............................................................2.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.1g

Egg yolk emulsion, 50%............................. 100.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

NaCl (0.9% solution) .................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, 50%, to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly.

Gently heat while stirring and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 100.0mL of sterile egg

yolk emulsion, 50%. Mix thoroughly. Pour into ster

ile Petri dishes in 15.0mL volumes.

Use: For the isolation and cultivation of Clostridium

perfringens in foods.