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Medium for Aciduric, Thermophilic Bacillus Strains

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Aciduric,

Thermophilic Bacillus Strains

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A................................................... 500.0mL

Solution B ................................................... 500.0mL

pH 4.3 ± 0.2 at 25°C

Solution A:

Composition per 500.0mL:

KH2PO4...............................................................3.0g

Glucose ...............................................................1.0g

Starch ..................................................................1.0g

Tryptone..............................................................1.0g

Yeast extract........................................................1.0g

MgSO4·7H2O ......................................................0.5g

CaCl2·2H2O.......................................................0.25g

(NH4)2SO4...........................................................0.2g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

500.0mL. Mix thoroughly. Adjust pH to 4.3. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Solution B:

Composition per 500.0mL:

Agar ..................................................................20.0g

Preparation of Solution B: Add agar to distilled/

deionized water and bring volume to 500.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine

500.0mL of solution A and 500.0mL of solution B.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation of aciduric, thermophilic

Bacillus strains.