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Membrane Clostridium perfringens Medium (m-CP Medium)
[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]
Membrane Clostridium perfringens
Medium
(m-CP Medium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1040mL:
Tryptose ............................................................30.0g
Yeast extract......................................................20.0g
Agar ..................................................................15.0g
Sucrose................................................................5.0g
L-Cysteine·HCl·H2O ...........................................1.0g
MgSO4·7H2O ......................................................0.1g
Bromcresol Purple ............................................0.04g
Phenolphthalein solution............................... 20.0mL
Indoxyl-β-D-glucoside solution ...................... 8.0mL
Selective supplement solution ........................ 8.0mL
Ferric chloride solution................................... 4.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Selective Supplement Solution:
Composition per 8.0mL:
D-Cycloserine......................................................0.8g
Polymyxin B sulfate ......................................50.0mg
Preparation of Selective Supplement Solu
tion: Add componetns to distilled/deionized water
and bring volume to 8.0mL. Mix thoroughly. Filter
sterilize.
Indoxyl-β-D-glucoside Solution:
Composition per 10.0mL:
Indoxyl-β-D-glucoside ...................................75.0mg
Preparation of Indoxyl-β-D-glucoside Solu
tion: Add indoxyl-β-D-glucoside to distilled/deion
ized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Ferric Chloride Solution:
Composition per 10.0mL:
FeCl3·6H2O.......................................................0.45g
Preparation of Ferric Chloride Solution: Add
ferric chloride to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Phenolphthalein Solution:
Composition per 20.0mL:
Phenolphthalein biphosphate
tetrasodium salt ..........................................0.15g
Preparation of Phenolphthalein Solution:
Add phenolphthalein biphosphate tetrasodium salt to
distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept selective supplement solution, phenolophthalein
solution, ferric chloride solution, and indoxyl-β-D
glucoside to distilled/deionized water and bring vol
ume to 1.0L. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 8.0mL selec
tive supplement solution, 20.0mL phenolophthalein
solution, 4.0mL ferric chloride solution, and 8.0mL
indoxyl-β-D-glucoside. Mix thoroughly. Pour into
sterile Petri dishes or aseptically distribute into ster
ile tubes.
Use: For the rapid isolation and presumptive identi
fication of Clostridium perfringens from food and
water samples. A selective and chromogenic medium
for the presumptive identification of Clostridium
perfringens, especially from water samples. Recom
mended in European Council Directive 98/83/EC for
testing the quality of water intended for human con
sumption.C. perfringens colonies have a characteris
tic opaque yellow appearance. Most other Clostridi
um spp. will appear as either purple colonies, due to
the lack of sucrose fermentation, or blue/green colo-
nies where the organism is still cleaving indoxyl-β-
D-glucoside and also fermenting sucrose.