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Middlebrook 7H9 Broth with Middlebrook OADC Enrichment

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H9 Broth with

Middlebrook OADC Enrichment

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4.............................................................2.5g

KH2PO4...............................................................1.0g

Monosodium glutamate ......................................0.5g

(NH4)2SO4 ..........................................................0.5g

Sodium citrate.....................................................0.1g

MgSO4·7H2O....................................................0.05g

Ferric ammonium citrate...................................0.04g

CuSO4·5H2O....................................................1.0mg

Pyridoxine........................................................1.0mg

ZnSO4·7H2O ....................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·2H2O .....................................................0.5mg

Middlebrook OADC enrichment ................ 100.0mL

Glycerol .......................................................... 2.0mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Middlebrook OADC Enrichment:

Composition per 100.0mL:

Bovine albumin fraction V .................................5.0g

Glucose ...............................................................2.0g

NaCl..................................................................0.85g

Oleic acid ..........................................................0.05g

Catalase............................................................4.0mg

Source: This enrichment is available as a prepared

enrichment from BD Diagnostic Systems.

Preparation of Middlebrook OADC Enrich

ment: Add components to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add glycerol to

900.0mL of distilled/deionized water and add re

maining components, except Middlebrook OADC

enrichment. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C. Aseptically add 100.0mL

of sterile Middlebrook OADC enrichment. Mix thor

oughly. Distribute into sterile tubes or flasks.

Use: For the isolation, cultivation, and maintenance

of Mycobacterium species, including Mycobacte

rium tuberculosis. Also used for determining the

antimicrobial susceptibility of mycobacteria.

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Middlebrook 7H9 Broth with Middlebrook OADC Enrichment