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Middlebrook 7H10 Agar with Middle brook OADC Enrichment and Hemin (Hemin Medium for Mycobacterium)

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H10 Agar with Middle

brook OADC Enrichment and Hemin

(Hemin Medium for Mycobacterium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Na2HPO4.............................................................1.5g

KH2PO4...............................................................1.5g

(NH4)2SO4 ..........................................................0.5g

L-Glutamic acid...................................................0.5g

Sodium citrate.....................................................0.4g

Ferric ammonium citrate...................................0.04g

MgSO4·7H2O..................................................0.025g

ZnSO4·7H2O ....................................................1.0mg

CuSO4·5H2O....................................................1.0mg

Pyridoxine........................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·2H2O .....................................................0.5mg

Malachite Green.............................................0.25mg

Middlebrook OADC enrichment ................ 100.0mL

Glycerol .......................................................... 5.0mL

Hemin solution................................................ 3.9mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Middlebrook OADC Enrichment:

Composition per 100.0mL:

Bovine albumin fraction V .................................5.0g

Glucose ...............................................................2.0g

NaCl..................................................................0.85g

Oleic acid..........................................................0.05g

Catalase............................................................4.0mg

Preparation of Middlebrook OADC Enrich

ment: Add components to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Hemin Solution:

Composition per 100.0mL:

Hemin .................................................................1.0g

NaOH (1N solution)...................................... 20.0mL

Preparation of Hemin Solution: Add hemin to

20.0mL of 1N NaOH solution. Mix thoroughly. Bring

volume to 100.0mL with distilled/deionized water.

Preparation of Medium: Add glycerol to

891.1mL of distilled/deionized water and add re

maining components, except Middlebrook OADC

enrichment. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C. Aseptically add 100.0mL

of sterile Middlebrook OADC enrichment. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the isolation, cultivation, and maintenance

of Mycobacterium species, including Mycobacte

rium tuberculosis. For the cultivation and mainte

nance of Mycobacterium haemophilum. Also used

for determining the antimicrobial susceptibility of

mycobacteria.

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Middlebrook 7H10 Agar with Middle brook OADC Enrichment and Hemin (Hemin Medium for Mycobacterium)