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Motility Indole Ornithine Medium (MIO Medium) (BAM M99)

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Indole Ornithine Medium

(MIO Medium)

(BAM M99)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone............................................................10.0g

Peptone..............................................................10.0g

L-Ornithine·HCl ..................................................5.0g

Yeast extract........................................................3.0g

Agar ....................................................................2.0g

Glucose ...............................................................1.0g

Bromcresol Purple ............................................0.02g

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the differentiation of Gram-negative

enteric bacteria based on their motility, indole pro

duction, and ornithine decarboxylase activity.