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Motility Medium S

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Medium S

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion......................500.0g

Gelatin...............................................................30.0g

Enzymatic hydrolyzate of protein.....................10.0g

NaCl....................................................................5.0g

K2HPO4...............................................................2.0g

KNO3 ..................................................................2.0g

Agar ....................................................................1.0g

2,3,5-Triphenyltetrazolium

chloride solution..................................... 10.0mL

pH 7.2 ± 0.2 at 25°C

2,3,5-Triphenyltetrazolium Chloride Solution:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium chloride ...................0.1g

Preparation of 2,3,5-Triphenyltetrazolium

Chloride Solution: Add 2,3,5-triphenyltetrazoli

um chloride to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept 2,3,5-triphenyltetrazolium chloride solution, to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat while stirring

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 60°C. Aseptically add

10.0mL of the sterile 2,3,5-triphenyltetrazolium

chloride solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes. Keep at 4°–8°C until used.

Use: For the determination of bacterial motility.