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Motility Nitrate Medium, Buffered (BAM M102)

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

Motility Nitrate Medium, Buffered

(BAM M102)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone................................................................5.0g

Galactose.............................................................5.0g

Agar ....................................................................3.0g

Beef extract.........................................................3.0g

Na2HPO4 .............................................................2.5g

KNO3 ..................................................................1.0g

Glycerin .......................................................... 5.0mL

pH 7.3 ± 0.1 at 25°C

Preparation of Medium: Add components, ex

cept agar, to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat until

dissolved. Add agar. Gently heat until boiling. Dis

tribute into tubes in 11.0mL volumes. Autoclave for

15 min at 15 psi pressure–121°C. If not used within

4 hr, heat tubes to 100°C for 10 min.

Use: For the cultivation and observation of the

motility of Clostridium perfringens.