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MRS Agar with Cysteine (LMG Medium 118)

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Agar with Cysteine

(LMG Medium 118)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Peptone..............................................................10.0g

Agar ..................................................................12.0g

Beef extract.........................................................5.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................2.0g

Diammonium hydrogen citrate ...........................2.0g

MgSO4·7H2O ......................................................0.2g

MnSO4·4H2O .................................................38.0mg

L-Cysteine solution ....................................... 10.0mL

Tween 80......................................................... 1.0mL

pH 6.5 ± 0.2 at 25°C

L-Cysteine Solution:

Composition per 10.0mL:

L-Cysteine ...........................................................0.5g

Preparation of L-Cysteine Solution: Add 0.1g

of L-cysteine to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept L-cysteine solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Ad

just pH to 6.5. Gently heat and bring to boiling. Dis

tribute into tubes or flasks. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°C. Aseptically

add 10.0mL of sterile L-cysteine solution. Mix thor

oughly. Aseptically pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the isolation and cultivation of Leuconos

toc mesenteroides and other lactic acid bacteria.