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MRS Agar with Tomato Juice pH 5.2 (LMG Medium 248)

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

MRS Agar with Tomato Juice pH 5.2

(LMG Medium 248)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Peptone .............................................................10.0g

Agar ..................................................................12.0g

Beef extract.........................................................5.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................2.0g

Diammonium hydrogen citrate...........................2.0g

MgSO4·7H2O......................................................0.2g

MnSO4·4H2O.................................................38.0mg

Tomato juice ............................................... 100.0mL

Tween 80......................................................... 1.0mL

pH 5.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 5.2 Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Lactobacil

lus kunkeei.