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MRS with Fermented Wort

[所属分类:培养基配方] [发布时间:2021-12-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A................................................... 800.0mL

Solution B ................................................... 200.0mL

pH 6.2 ± 0.2 at 25°C

Solution A:

Composition per liter:

Glucose .............................................................20.0g

Peptone..............................................................10.0g

Agar ..................................................................10.0g

Beef extract.........................................................8.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................4.0g

K2HPO4...............................................................2.0g

Triammonium citrate ..........................................2.0g

MgSO4·7H2O ......................................................0.2g

MnSO4·4H2O ....................................................0.05g

Sorbitan monooleate ....................................... 1.0mL

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C.

Solution B:

Composition per liter:

Malt extract.......................................................15.0g

Maltose............................................................12.75g

Dextrin ..............................................................2.75g

Glycerol ............................................................2.35g

K2HPO4...............................................................1.0g

NH4Cl .................................................................1.0g

Pancreatic digest of gelatin...............................0.78g

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to room temperature. Inoculate with Saccharomyces

cerevisiae to allow wort to ferment. Incubate for 24–

48 hr. Allow to cool to room temperature. Filter

through Whatman filter paper to remove solids. Filter

sterilize.

Preparation of Medium: Aseptically combine

800.0mL of sterile solution A and 200.0mL of sterile

solution B. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of yeasts and fungi.

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MRS with Fermented Wort