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Potassium Cyanide Broth (BAM M126)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potassium Cyanide Broth

(BAM M126)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4...........................................................5.64g

NaCl....................................................................5.0g

Proteose peptone No. 3.......................................3.0g

KH2PO4...........................................................0.225g

KCN solution................................................ 15.0mL

pH 7.6 ± 0.2 at 25°C

KCN Solution:

Composition per 100.0mL:

KCN....................................................................0.5g

Preparation of KCN Solution: Add KCN to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly.

Caution: Cyanide is toxic. Do not pipet by mouth.

Use protective equipment/gloves when handling.

Preparation of Medium: Add components, ex

cept KCN solution, to distilled/deionized water and

bring volume to 985.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C. Aseptically add

15.0mL of KCN solution. Mix thoroughly. Distribute

into sterile screw-capped tubes or flasks in 1.0–

1.5mL volumes. Close caps tightly. Store at 5–8°C no

longer than 2 weeks before use.

Use: For the cultivation and differentiation of ure

ase-negative, Gram-negative enteric bacteria. Salmo

nella species and Shigella species are nonmotile in

this medium. Proteus species are motile in this medi

um.