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Potato Dextrose Adenine Agar (ATCC Medium 2204)

[所属分类:培养基配方] [发布时间:2021-12-10] [发布人:网站管理员2] [阅读次数:] [返回]

Potato Dextrose Adenine Agar

(ATCC Medium 2204)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Potatoes, infusion from............................... 500.0mL

Adenine solution........................................... 50.0mL

pH 5.6 ± 0.2 at 25°C

Potato Infusion:

Composition per 500.0mL:

Potatoes...........................................................300.0g

Preparation of Potato Infusion: Peel and dice

potatoes. Add 500.0mL of distilled/deionized water.

Gently heat and bring to boiling. Continue boiling for

30 min. Filter through cheesecloth. Reserve filtrate.

Adenine Solution:

Composition per 50.0mL:

Adenine...............................................................0.1g

Preparation of Adenine Solution: Add adenine

to distilled/deionized water and bring volume to

50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept adenine solution to distilled/deionized water and

bring volume to 950.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

50.0mL of sterile adenine solution. Mix thoroughly.

Pour into sterile Petri dishes or distriubte to sterile

tubes.

Use: For the cultivation of yeasts and molds.