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Pseudomonas Medium (ATCC Medium 609)

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas Medium

(ATCC Medium 609)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

K2HPO4.............................................................8.71g

Nitrilotriacetic acid ...........................................1.91g

Na2SO4..............................................................0.57g

MgSO4 ..............................................................0.25g

FeSO4...............................................................0.5mg

Ca(NO3)2..........................................................0.5mg

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add nitrilotriacetic

acid to approximately 500.0mL of distilled/deionized

water. Mix thoroughly. Adjust pH to 6.5. Add re

maining components. Bring volume to 1.0L with dis

tilled/deionized water. Gently heat and bring to

boiling. Distribute into tubes or flasks. Autoclave for

15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of

Pseudomonas species.