培养基配方

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Pseudomonas saccharophila Medium

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1015.0mL:

Agar ..................................................................20.0g

Na2HPO4.............................................................4.8g

KH2PO4...............................................................4.4g

NH4Cl .................................................................1.0g

MgSO4·7H2O......................................................0.5g

Solution A....................................................... 5.0mL

Solution B ..................................................... 10.0mL

Solution A:

Composition per 100.0mL:

Ferric ammonium citrate.....................................1.0g

CaCl2...................................................................0.1g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Solution B:

Composition per 100.0mL:

Sucrose..............................................................10.0g

Preparation of Solution B: Add sucrose to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept solution A and solution B, to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile solution A and sterile solution

B. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation and maintenance of

Pseudomonas saccharophila and other Pseudomo

nas species.