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Purple Broth

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

Purple Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin...............................10.0g

NaCl....................................................................5.0g

Bromcresol Purple ............................................0.02g

Carbohydrate solution................................... 20.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 20.0mL. For expensive carbohydrates,

5.0g may be used instead of 10.0g. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 980.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Distribute into tubes

containing an inverted Durham tube. Fill each tube

with 9.8mL of medium. Autoclave for 15 min at 15

psi pressure–121°C. Aseptically add 0.2mL of sterile

carbohydrate solution to each tube.

Use: For the preparation of liquid fermentation me

dia. Bacteria that can ferment the carbohydrate turn

the medium yellow.