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R2A Agar

[所属分类:培养基配方] [发布时间:2021-12-13] [发布人:网站管理员2] [阅读次数:] [返回]

R2A Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Yeast extract........................................................0.5g

Acid hydrolysate of casein..................................0.5g

Glucose ...............................................................0.5g

Soluble starch......................................................0.5g

K2HPO4...............................................................0.3g

Sodium pyruvate.................................................0.3g

Pancreatic digest of casein................................0.25g

Peptic digest of animal tissue............................0.25g

MgSO4, anhydrous..........................................0.024g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat with mixing and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Do not over

heat. Pour into sterile Petri dishes or leave in tubes.

Use: For use in standard methods for pour plate,

spread plate, and membrane filter analysis to enu

merate heterotrophic bacteria from waters.