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Reinforced Clostridial Medium with Sodium Lactate

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Reinforced Clostridial Medium

with Sodium Lactate

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................10.0g

Beef extract.......................................................10.0g

Glucose ...............................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

Sodium acetate....................................................3.0g

Soluble starch......................................................1.0g

L-Cysteine·HCl·H2O ...........................................0.5g

Agar ....................................................................0.5g

Sodium lactate (60% solution)...................... 15.0mL

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 10 psi pressure–115°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the nonselective cultivation and enumera

tion of Clostridium species, other anaerobes such as

lactobacilli, and facultative organisms from foods.