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Russell Double-Sugar Agar

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Proteose peptone No. 3 .....................................12.0g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................1.0g

Glucose ...............................................................1.0g

Phenol Red......................................................0.025g

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distribute

into tubes. Autoclave for 15 min at 15 psi pressure–

121°C. Allow tubes to cool in a slanted position.

Use: For the identification of Gram-negative enteric

bacilli based on their fermentation of glucose and

lactose. Bacteria that ferment both glucose and lac

tose produce a yellow slant and yellow butt. Bacteria

that ferment glucose but do not ferment lactose pro

duce a red slant and a yellow butt. Bacteria that fer

ment neither glucose nor lactose produce an un

changed pink-orange color.