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SA Agar, Modified (Lachica’s Medium)

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

SA Agar, Modified

(Lachica’s Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion......................500.0g

Agar ..................................................................15.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Amylose azure ....................................................3.0g

Ampicillin ......................................................0.01mg

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes.

Use: For the isolation and cultivation of Aeromonas

hydrophila from foods. Aeromonas hydrophila ap

pears as colonies surrounded by a light halo on a light

blue background.