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Salmonella Chromogenic Agar Base (OSCM)

[所属分类:培养基配方] [发布时间:2021-12-14] [发布人:网站管理员2] [阅读次数:] [返回]

Salmonella Chromogenic Agar Base

(OSCM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Chromogenic mix .............................................28.0g

Agar ..................................................................12.0g

Special Peptone.................................................10.0g

Salmonella selective supplement.................. 10.0mL

pH 7.2 ± 0.2 at 25°C

Salmonella Selective Supplement:

Composition per 10.0mL:

Cefulodin .......................................................12.0mg

Novobiocin.......................................................5.0mg

Preparation of Salmonella Selective Supple

ment: Add components to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to a

boil to dissolve the components. Do not hold at boil

ing. Do not autoclave. Cool to 50°C. Aseptically add

10.0mL Salmonella selective supplement. Mix thor

oughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the identification of Salmonella spp. based

upon the ability to utilize a specific chromogenic

substrate which allows differentiation of Salmonella

spp. from other members of the Enterobacteriaceae.

The medium combines two chromogens for the de

tection of Salmonella spp., 5-Bromo-6-Chloro-3-In

dolyl caprylate (Magenta-caprylate) and 5-Bromo-4-

chloro-3-indolyl β-D-galactopyranoside (X-gal). X

gal is a substrate for the enzyme β-D-galactosidase.

Hydrolysis of the chromogen, Mag-caprylate, by lac

tose negative Salmonella species results in magenta

colonies. The medium contains bile salts to inhibit

the growth of Gram-positive organisms and the addi

tion of the Salmonella selective supplement increases

the selectivity of the medium by inhibiting growth of

Proteus spp. and Pseudomonas spp.