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Salmonella Shigella Agar (SS Agar)

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Salmonella Shigella Agar

(SS Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.5g

Lactose..............................................................10.0g

Bile salts..............................................................8.5g

Na2S2O3 ..............................................................8.5g

Sodium citrate.....................................................8.5g

Beef extract.........................................................5.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue..............................2.5g

Ferric citrate........................................................1.0g

Neutral Red.....................................................0.025g

Brilliant Green ...............................................0.33mg

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Do not autoclave. Cool to 45°–50°C. Pour

into sterile Petri dishes in 20.0mL volumes. Allow

the surface of the plates to dry before inoculation.

Use: For the selective isolation and differentiation of

pathogenic enteric bacilli, especially those belonging

to the genus Salmonella. This medium is not recom

mended for the primary isolation of Shigella species.

Lactose-fermenting bacteria such as Escherichia coli

or Klebsiella pneumoniae appear as small pink or red

colonies. Lactose-nonfermenting bacteria—such as

Salmonella species, Proteus species, and Shigella

species—appear as colorless colonies. Production of

H2S by Salmonella species turns the center of the col

onies black.