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Seawater Agar with Fetal Calf Serum
[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]
Seawater Agar with Fetal Calf Serum
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ..................................................................20.0g
Beef extract.......................................................10.0g
Peptone .............................................................10.0g
Seawater...................................................... 750.0mL
Fetal calf serum........................................... 100.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept fetal calf serum, to tap water and bring volume
to 900.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 100.0mL
of sterile fetal calf serum warmed to 50°–55°C. Mix
thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation of Aeromonas species and
Vibrio salmonicida.