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Selection Agar II

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Selection Agar II

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium acetate..................................................40.0g

Agar ..................................................................25.0g

(NH4)2SO4 ..........................................................5.0g

Maltose ...............................................................2.5g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O ........................................................0.1g

Inositol .............................................................2.0mg

KI .....................................................................1.0mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyroxidine·HCl ................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

CuSO4·5H2O..................................................0.04mg

Folic acid ..........................................................2.0μg

Biotin ................................................................2.0μg

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the selective cultivation of Saccharomyces

cerevisiae.