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Selenite Broth Base, Mannitol

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4 ...........................................................10.0g

Peptone................................................................5.0g

Mannitol..............................................................4.0g

NaHSeO3·5H2O ..................................................4.0g

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Caution: Sodium selenite is toxic and a potential

teratogen and care must be taken to avoid inhalation

of the powdered dye, contact with the skin, or inges

tion, especially in pregnant laboratory workers.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat. Do not autoclave. Dis

tribute into sterile tubes in 10.0mL volumes. Sterilize

for 10 min at 0 psi pressure–100°C.

Use: For the isolation and cultivation of Salmonella

typhi and Salmonella paratyphi.