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Semi-Solid Medium, Modified (BAM M30c)

[所属分类:培养基配方] [发布时间:2021-12-15] [发布人:网站管理员2] [阅读次数:] [返回]

Semi-Solid Medium, Modified

(BAM M30c)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................10.0g

Lactalbumin hydrolysate.....................................5.0g

Yeast extract........................................................5.0g

NaCl....................................................................5.0g

Agar ....................................................................1.8g

α-Ketoglutaric acid.............................................1.0g

Na-pyruvate ........................................................0.5g

Na2S2O5 ..............................................................0.5g

NaCO3 ................................................................0.6g

Na-citrate ............................................................0.1g

Hemin................................................................0.01g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Cool to 50°C. Adjust pH to 7.4. Distribute into

screw-capped tubes in 10.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C.

Use: For the storage and preservation of Campylo

bacter spp.