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Seven H11 Agar (Selective 7H11 Agar)

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Seven H11 Agar

(Selective 7H11 Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Agar ..................................................................13.5g

KH2PO4...............................................................1.5g

Na2HPO4 .............................................................1.5g

Pancreatic digest of casein..................................1.0g

NaCl..................................................................0.85g

Monosodium glutamate ......................................0.5g

(NH4)2SO4...........................................................0.5g

Sodium citrate.....................................................0.4g

MgSO4·7H2O ....................................................0.05g

Ferric ammonium citrate...................................0.04g

CuSO4·5H2O ....................................................1.0mg

Pyridoxine........................................................1.0mg

ZnSO4·7H2O ....................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·2H2O......................................................0.5mg

Malachite Green.............................................0.25mg

Glycerol .......................................................... 5.0mL

Middlebrook OADC enrichment ................ 100.0mL

Antibiotic inhibitor ....................................... 10.0mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems.

Middlebrook OADC Enrichment:

Bovine albumin fraction V .................................5.0g

Glucose ...............................................................2.0g

NaCl..................................................................0.85g

Catalase............................................................3.0mg

Oleic acid ...................................................... 0.06mL

Preparation of Middlebrook OADC Enrich

ment: Add components to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Antibiotic Inhibitor:

Composition per 10.0mL:

Carbenicillin......................................................0.05g

Trimethoprim lactate.........................................0.02g

Amphotericin B ................................................0.01g

Polymyxin B ..............................................200,000U

Preparation of Antibiotic Inhibitor: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add glycerol to

900.0mL of distilled/deionized water. Mix thorough

ly. Add remaining components, except Middlebrook

OADC enrichment and antibiotic inhibitor. Mix thor

oughly. Gently heat. Do not boil. Autoclave for 10

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Aseptically add 100.0mL of sterile Middlebrook

OADC enrichment and 10.0mL of sterile antibiotic

solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes.

Use: For the isolation and cultivation of Mycobacte

rium species from specimens with a mixed flora.

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Seven H11 Agar (Selective 7H11 Agar)