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SF Broth (Streptococcus faecalis Broth)

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

SF Broth

(Streptococcus faecalis Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Glucose ...............................................................5.0g

NaCl....................................................................5.0g

K2HPO4...............................................................4.0g

KH2PO4...............................................................1.5g

NaN3 ...................................................................0.5g

Bromcresol Purple ..........................................0.032g

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of group

D enterococci (Streptococcus faecalis and Strepto

coccus faecium) from group D nonenterococci and

from other Streptococcus species. Group D entero

cocci turn the medium turbid and yellow-brown.