- 定做培养基/定制培养基
- 2020年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
培养基配方
您现在的位置: 网站首页 >> 培养基配方
Shigella Broth (BAM M136)
[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]
Shigella Broth
(BAM M136)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein................................20.0g
NaCl....................................................................5.0g
K2HPO4...............................................................2.0g
KH2PO4...............................................................2.0g
Glucose ...............................................................1.0g
Novobiocin solution...................................... 11.1mL
Tween 80......................................................... 1.5mL
pH 7.0 ± 0.2 at 25°C
Novobiocin Solution:
Composition per liter:
Novobiocin........................................................0.05g
Preparation of Novobiocin Solution: Add no
vobiocin to distilled/deionized water and bring vol
ume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept novobiocin solution, to distilled/deionized water
and bring volume to 988.9mL. Mix thoroughly. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptical
ly add sterile novobiocin solution. Mix thoroughly.
Aseptically distribute into sterile tubes.
Use: For the isolation and cultivation of Shigella
species from food.