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Sodium Dodecyl Sulfate Polymyxin Sucrose Agar

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Sodium Dodecyl Sulfate

Polymyxin Sucrose Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Agar ..................................................................15.0g

Sucrose..............................................................15.0g

Proteose peptone...............................................10.0g

Beef extract.........................................................5.0g

Sodium lauryl sulfate..........................................1.0g

Bromthymol Blue .............................................0.04g

Cresol Red.........................................................0.04g

Polymyxin B solution ................................... 10.0mL

pH 7.6 ± 0.2 at 25°C

Polymyxin B Solution:

Composition per 10.0mL:

Polymyxin B sulfate...................................100,000U

Preparation of Polymyxin B Solution: Add

polymyxin B sulfate to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept polymyxin B solution, to distilled/deionized wa

ter and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile polymyxin B solution. Mix

thoroughly. Pour immediately into sterile Petri dishes

or distribute into sterile tubes.

Use: For the isolation and cultivation of Vibrio spe

cies from foods.